Kinetic Measurement of T 4 Following Column Chromatography

Column Liquid Chromatography

Phosphorus assay in column chromatography.

Malerials-30 per cent hydrogen peroxide (Baker and Adamson, phosphorus-free) and the ammonium molybdate, (NHJ6lMo7024.4Hz0, wereused. The Fiske and SubbaRow (I) reagent was prepared by adding 0.5 gm. of purified (1) 1-amino-2naphthol-4-sulfonic acid (Eastman Organic Chemicals) with mechanical stirring to 200 ml. of freshly prepared 15 per cent sodium bisulfite (anhydrous), followed by 1.0 gm. o...

Packed Column Supercritical Fluid Chromatography

Nicole Riddell (2017): Packed Column Supercritical Fluid Chromatography: Applications in Environmental Chemistry. Örebro Studies in Chemistry 19. Although gas and liquid chromatography have emerged as dominant separation techniques in environmental analytical chemistry, these methods do not allow for the concurrent analysis of chemically diverse groups of persistent organic pollutants (POPs). T...

Separation of aflatoxins by column chromatography.

Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...

Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography

As column volumes continue to decrease, extra-column band broadening has become an increasingly important consideration when determining column performance. Combined contributions due to the injector and connecting tubing in a capillary LC system were measured and found to be larger than expected by Taylor-Aris theory. Variance from sigma-type and tau-type broadening was isolated from eluted pe...